3 years ago

A Novel N‐Substituted Pyrrole Based Surface Modification for Biosensing

Immobilization of proteins, mostly antigens, antibodies or enzymes on a solid surface, is one of the most important steps in the development of every biosensor. Different chemical methods as well as site‐directed strategies have been developed in order to obtain highest functionality and stability for the sensoric device. In the present work, a novel method for the immobilization of biomolecules on a surface plasmon resonance (SPR) device is described. New N‐substituted pyrroles are synthesized and deposited on a gold surface using electrochemical polymerization. To evaluate the established four different surfaces, the binding behavior between a fusion protein consisting of the lectin concanavalin A (ConA) as well as streptavidin (Sav) binding domains and the sugar mannan, which has been chemically coupled to the poly‐pyrrole (PPy) surface is investigated. A pyrrole‐N‐C16/pyrrole co‐polymer surface as well as a pyrrol‐1‐yl hexanoic acid/pyrrole (PHCP) surface give best results.

Publisher URL: https://onlinelibrary.wiley.com/doi/abs/10.1002/pssa.201800030

DOI: 10.1002/pssa.201800030

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