3 years ago

The N-terminal domain of <i>Mycobacterium tuberculosis</i> PPE17 (Rv1168c) protein plays a dominant role in inducing antibody responses in active TB patients

Sangita Mukhopadhyay, Niteen Pathak, Gourango Pradhan, Philip Raj Abraham, Gaddam Sumanlatha

by Philip Raj Abraham, Niteen Pathak, Gourango Pradhan, Gaddam Sumanlatha, Sangita Mukhopadhyay

The PPE (proline-proline-glutamic acid) proteins of Mycobacterium tuberculosis are characterized by a conserved N-terminal domain of approximately 180 amino acids and variable C-terminal domain. Since last decade, these proteins have gained much importance in the serodiagnosis of tuberculosis (TB) as they act as a source of antigenic variation. We have demonstrated earlier that one of the PPE proteins PPE17 (Rv1168c) induces strong B-cell and T-cell responses in active TB disease and also displays a higher antibody titer compared to immunodominant antigens such as ESAT-6, Hsp60 and PPD. However, the immunodominant domain of PPE17 (N-terminal or C-terminal) was not examined in detail. In the present study, we observed that antibody responses elicited in TB patients were directed mostly towards the N-terminal domain of PPE17 (N-PPE17). The antibody generated against N-PPE17 in TB patients did not significantly cross-react with N-terminal domains of other PPE proteins used in this study. Our data suggest that the N-terminal domain of PPE17 protein is immunodominant and could be used as a better serodiagnostic marker than the full-length PPE17 protein.

Publisher URL: http://journals.plos.org/plosone/article

DOI: 10.1371/journal.pone.0179965

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