Angewandte Chemie - International Edition
Angewandte Chemie - International Edition
5 years ago

Efficient Gene Transfection through Inhibition of β-Sheet (Amyloid Fiber) Formation of a Short Amphiphilic Peptide by Gold Nanoparticles

Efficient Gene Transfection through Inhibition of β-Sheet (Amyloid Fiber) Formation of a Short Amphiphilic Peptide by Gold Nanoparticles
Carsten Schmuck, Shirley Knauer, Sandra Bäcker, Elio Zellermann, Krishnananda Samanta, Poulami Jana
The effect of citrate-stabilized gold nanoparticles (AuNPs) on the secondary structure of an artificial β-sheet-forming cationic peptide has been studied. The AuNPs inhibited β-sheet formation and led to fragmented fibrils and spherical oligomers with assembled AuNPs on their surface. Besides this structural change, the functional properties of the peptide are also different. Whereas the peptide was unable to act as a vector for gene delivery, formation of a complex with AuNPs allowed successful gene delivery into cells. Call the tune: Negatively charged gold nanoparticles (AuNPs) have been found to inhibit the ability of cationic amphiphilic peptide 1 to form β-sheet structures and also induce disruption of the aggregates. Furthermore, they also tune the functional properties of the peptide, with the complex being an efficient gene transfection vector whereas the peptide by itself is not.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/anie.201700713

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