5 years ago

Myc Regulates Chromatin Decompaction and Nuclear Architecture during B Cell Activation.

Jung S, Dose M, Stavreva D, Floer M, Hager G, Johnson SM, Dong P, Nimura K, Resch W, Phair RD, Gregory C, Tomassoni Ardori F, Batchelor E, Cosma MP, Kieffer-Kwon KR, Aiden EL, Tessarollo L, Finn E, Baranello L, Xu J, Lakadamyali M, St Hilaire BG, Levens D, Stevens E, Zheng Y, Ricci MA, Mathe E, Casellas R, Liu Z, Dubois W, McAndrew M, Pekowska A, Casellas A, Nelson S, Shroff H, Huang SC, Rao SSP, Misteli T
50 years ago, Vincent Allfrey and colleagues discovered that lymphocyte activation triggers massive acetylation of chromatin. However, the molecular mechanisms driving epigenetic accessibility are still unknown. We here show that stimulated lymphocytes decondense chromatin by three differentially regulated steps. First, chromatin is repositioned away from the nuclear periphery in response to global acetylation. Second, histone nanodomain clusters decompact into mononucleosome fibers through a mechanism that requires Myc and continual energy input. Single-molecule imaging shows that this step lowers transcription factor residence time and non-specific collisions during sampling for DNA targets. Third, chromatin interactions shift from long range to predominantly short range, and CTCF-mediated loops and contact domains double in numbers. This architectural change facilitates cognate promoter-enhancer contacts and also requires Myc and continual ATP production. Our results thus define the nature and transcriptional impact of chromatin decondensation and reveal an unexpected role for Myc in the establishment of nuclear topology in mammalian cells.

Publisher URL: https://www.ncbi.nlm.nih.gov/pubmed/28803781

DOI: PubMed:28803781

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.