ACS Omega
ACS Omega
3 years ago

Hesperidin Targets Sterol C-24 Reductase to Fight against Leishmaniasis.

Hesperidin Targets Sterol C-24 Reductase to Fight against Leishmaniasis.
Shams Tabrez, Fazlur Rahman, Rahat Ali, Sajjadul Kadir Akand, Mohammed A. Alaidarous, Saeed Banawas, Abdul Aziz Bin Dukhyil, Abdur Rub
Hesperidin, a naturally occurring flavanoid, is present in citrus family of fruits. It was found effective against an array of pathogens including fungi, bacteria, viruses, and protozoa. Here, we evaluated its antileishmanial activity and possible mechanism of action through different in vitro and in silico experiments. It inhibited the growth and proliferation of the parasites significantly with a IC50 value of 1.019 ± 0.116 mM in vitro. It also reduced the growth of intra-macrophagic amastigotes with a IC50 value of 0.2858 ± 0.01398 mM. It induced the reactive oxygen species (ROS) in parasites in a dose-dependent manner. Through 2,7-dichloro dihydro fluorescein diacetate (H2DCFDA) staining, it was observed that around 96.9% parasites were ROS positive at 2.0 mM concentration of hesperidin. The ROS generated led to the apoptosis of parasites in a dose-dependent manner as observed by annexin/PI staining. Molecular docking with one of the very important and unique drug-targets of Leishmania donovani sterol C-24 reductase resulted in its significant inhibition. Here, we for the first time showed that hesperidin induced the antileishmanial response through the induction of apoptosis and inhibition of sterol C-24 reductase. Our study will be helpful in the development of a cost-effective antileishmanial lead with higher efficacy. This article has not yet been cited by other publications.

Publisher URL: http://dx.doi.org/10.1021/acsomega.0c05858

Open URL: https://pubs.acs.org/doi/pdf/10.1021/acsomega.0c05858

DOI: 10.1021/acsomega.0c05858

Open access
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