Conditional Network Assembly and Targeted Protein Retention via Environmentally Responsive, Engineered β-Roll Peptides

5 years ago

Conditional Network Assembly and Targeted Protein Retention via Environmentally Responsive, Engineered β-Roll Peptides

Scott Banta, Sarah J. Yang, Beyza Bulutoglu

Stimulus-responsive biomaterials have applications in many areas of biotechnology, such as tissue engineering, drug delivery, and bioelectrocatalysis. The intrinsically disordered repeat-in-toxin (RTX) domain is a conformationally dynamic peptide that gains β-roll secondary structure when bound to calcium ions. A smart hydrogel platform was constructed by genetically fusing two rationally designed mutant RTX domains: first, a mutant peptide with hydrophobic interfaces capable of calcium-dependent network assembly, and second, another mutant that conditionally binds the model target protein lysozyme. In this way, the calcium-induced control over the secondary structure of the β-roll peptide was exploited to regulate both the cross-linking and lysozyme-binding functionalities. The constructed biomaterial exhibited calcium-dependent gelation and target molecule retention, and erosion experiments showed that β-roll peptides with a higher affinity for lysozyme produced more robust hydrogel networks. This work demonstrates the use of RTX domains for introducing two useful features simultaneously, network cross-linking and target protein binding, and that the calcium-dependent regulation of these systems can be useful for controlling bulk self-assembly and controlled release capabilities.

Publisher URL: http://dx.doi.org/10.1021/acs.biomac.7b00457

DOI: 10.1021/acs.biomac.7b00457