Divergent roles for Ly6C<sup>+</sup>CCR2<sup>+</sup>CX3CR1<sup>+</sup> inflammatory monocytes during primary or secondary infection of the skin with the intra-phagosomal pathogen <i>Leishmania major</i>
by Audrey Romano, Matheus B. H. Carneiro, Nicole A. Doria, Eric H. Roma, Flavia L. Ribeiro-Gomes, Ehud Inbar, Sang Hun Lee, Jonatan Mendez, Andrea Paun, David L. Sacks, Nathan C. Peters
Inflammatory monocytes can be manipulated by environmental cues to perform multiple functions. To define the role of monocytes during primary or secondary infection with an intra-phagosomal pathogen we employed Leishmania major-red fluorescent protein (RFP) parasites and multi-color flow cytometry to define and enumerate infected and uninfected inflammatory cells in the skin. During primary infection, infected monocytes had altered maturation and were the initial mononuclear host cell for parasite replication. In contrast, at a distal site of secondary infection in mice with a healed but persistent primary infection, this same population rapidly produced inducible nitric oxide synthase (iNOS) in an IFN-γ dependent manner and was critical for parasite killing. Maturation to a dendritic cell-like phenotype was not required for monocyte iNOS-production, and enhanced monocyte recruitment correlated with IFN-γ dependent cxcl10 expression. In contrast, neutrophils appeared to be a safe haven for parasites in both primary and secondary sites. Thus, inflammatory monocytes play divergent roles during primary versus secondary infection with an intra-phagosomal pathogen.Publisher URL: http://journals.plos.org/plosone/article
DOI: 10.1371/journal.ppat.1006479
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