5 years ago

Protein-bound polyphenols create “ghost” band artifacts during chemiluminescence-based antigen detection [version 2; referees: 2 approved, 1 approved with reservations]

Mary Ann Lila, Scott Laster, Nathalie Plundrich, Edward Foegeding
Antigen detection during Western blotting commonly utilizes a horseradish peroxidase-coupled secondary antibody and enhanced chemiluminescent substrate. We utilized this technique to examine the impact of green tea-derived polyphenols on the binding of egg white protein-specific IgE antibodies from allergic human plasma to their cognate antigens. Our experiments unexpectedly showed that green tea-derived polyphenols, when stably complexed with egg white proteins, caused “ghost” band formation in the presence of horseradish peroxide. This study suggests that caution should be taken when evaluating polyphenol-bound proteins by enhanced chemiluminescence Western blotting using horseradish peroxidase and demonstrates that protein-bound polyphenols can be a source of “ghost” band artifacts on Western blots.

Publisher URL: https://f1000research.com/articles/6-254/v2

DOI: 10.12688/f1000research.10622.2

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