4 years ago

Distinguishing Individual DNA Bases in a Network by Non-Resonant Tip-Enhanced Raman Scattering

Distinguishing Individual DNA Bases in a Network by Non-Resonant Tip-Enhanced Raman Scattering
Zhenchao Dong, Xianbiao Zhang, Yang Zhang, Yi Luo, Huifang Wang, Chunrui Hu, Rui Zhang, Yao Zhang, Song Jiang
The importance of identifying DNA bases at the single-molecule level is well recognized for many biological applications. Although such identification can be achieved by electrical measurements using special setups, it is still not possible to identify single bases in real space by optical means owing to the diffraction limit. Herein, we demonstrate the outstanding ability of scanning tunneling microscope (STM)-controlled non-resonant tip-enhanced Raman scattering (TERS) to unambiguously distinguish two individual complementary DNA bases (adenine and thymine) with a spatial resolution down to 0.9 nm. The distinct Raman fingerprints identified for the two molecules allow to differentiate in real space individual DNA bases in coupled base pairs. The demonstrated ability of non-resonant Raman scattering with super-high spatial resolution will significantly extend the applicability of TERS, opening up new routes for single-molecule DNA sequencing. A or T? TERS can tell: Real-space chemical recognition of single DNA bases in hydrogen-bonded networks was realized for the first time. This was achieved by using the recently developed sub-nanometer-resolved tip-enhanced Raman scattering (TERS) technique based on ultrahigh-vacuum and low-temperature scanning tunneling microscopy.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/anie.201702263

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