Fertility and Sterility
Fertility and Sterility
5 years ago

Impact of 3.5% O2 culture on embryo development and clinical outcomes: a comparative study

To evaluate the effect of culturing human embryos in vitro in 3.5% oxygen (O2) concentration. Design Comparative study. Setting Private IVF center. Patient(s) The study included 558 women in two groups. Intervention(s) After intracytoplasmic sperm injection (ICSI), women's oocytes were assigned to undergo cultivation in either 3.5% O2 concentration (intervention) or 5% O2 level (control group), continuously, from day 0 through day 5 or 6. Main Outcome Measure(s) Clinical pregnancy rate (PR) after ET. Result(s) There were significantly higher fertilization and cleavage rates in the 3.5% O2 group (odds ratio [OR] 1.72, 95% confidence interval [CI] 1.53–1.93) and (OR 3.74, 95% CI 2.30–6.07) than in the 5% O2 group. The compaction rate on day 3, and the number of formed, high-quality and cryopreserved blastocysts on day 5 were significantly lower in 3.5% O2 than in 5% O2 concentration ([OR 0.81, 95% CI 0.69–0.91], [OR 0.40, 95% CI 0.36–0.46], [OR 0.32, 95% CI 0.28–0.37] and [OR 0.47, 95% CI 0.40–0.54]), respectively. Culturing embryos in 3.5% O2 concentration led to significantly lower rates of biochemical pregnancy, clinical PR, and implantation ([OR 0.66, 95% CI 0.47–0.92], [OR 0.60, 95% CI 0.43–0.84] and [OR 0.61, 95% CI 0.46–0.81]), respectively. Conclusion(s) Culturing human embryos, continuously from day 0 to 5 or 6, in 3.5% O2 concentration is associated with significantly lower blastocyst formation rate and clinical outcomes parameters, but rather with significantly higher rates of fertilization and cleavage. Whether these findings hold true for other patient populations and culture media brands remain unknown.

Publisher URL: www.sciencedirect.com/science

DOI: S0015028217305332

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.