3 years ago

RUNX2 Induction during Differentiation of Wharton's Jelly Mesenchymal Stem Cells to Osteoblasts is Regulated by JARID1B Histone Demethylase

RUNX2 Induction during Differentiation of Wharton's Jelly Mesenchymal Stem Cells to Osteoblasts is Regulated by JARID1B Histone Demethylase
José Lattus, Margarita Carrasco, Verónica Palma, Martín Montecino, José Palma, Lorena Díaz, Francisco Bustos, Catalina P. Prieto, Hugo Sepúlveda
Novel bone regeneration approaches aim to obtain immature osteoblasts from somatic stem cells. Umbilical cord Wharton's jelly mesenchymal stem cells (WJ-MSC) are an ideal source for cell therapy. Hence, the study of mechanisms involved in WJ-MSC osteoblastic differentiation is crucial to exploit their developmental capacity. Here, we have assessed epigenetic control of the RUNX2 osteogenic master regulator gene in WJ-MSC. We present evidence indicating that modulation of RUNX2 expression through preventing JARID1B histone demethylase activity is relevant to enhance WJ-MSC osteoblastic potential. Hence, JARID1B loss of function in WJ-MSC results in increased RUNX2/p57 expression. Our data highlights JARID1B activity as a novel target to modulate WJ-MSC osteoblastic differentiation with potential applications in bone tissue engineering. This article is protected by copyright. All rights reserved. The epigenetic repressor JARID1B is enriched at the RUNX2 promoter in WJ-MSCs, resulting in a weak/transient induction of endogenous RUNX2 expression thus restricting the osteoblast differentiation capability in intact cells. Gene silencing or pharmacological inhibition of JARID1B increases the RUNX2 levels during WJ-MSCs osteoblast differentiation modifying the epigenetic state of RUNX2 promoter, introducing new possibilities for bone tissue engineering.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/stem.2704

You might also like
Never Miss Important Research

Researcher is an app designed by academics, for academics. Create a personalised feed in two minutes.
Choose from over 15,000 academics journals covering ten research areas then let Researcher deliver you papers tailored to your interests each day.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.