3 years ago

Unveiling the biotransformation mechanism of indole in a Cupriavidus sp. strain

Unveiling the biotransformation mechanism of indole in a Cupriavidus sp. strain
Ping Xu, Hongzhi Tang, Weiwei Wang, Jiti Zhou, Ziyan Liu, Yuanyuan Qu, Qiao Ma
Indole, an important signaling molecule as well as a typical N-heterocyclic aromatic pollutant, is widespread in nature. However, the biotransformation mechanisms of indole are still poorly studied. Here, we sought to unlock the genetic determinants of indole biotransformation in strain Cupriavidus sp. SHE based on genomics, proteomics, and functional studies. A total of 177 proteins were notably altered (118 up- and 59 down-regulated) in cells grown in indole mineral salt medium when compared with that in sodium citrate medium. RT-qPCR and gene knockout assays demonstrated that an indole oxygenase gene cluster was responsible for the indole upstream metabolism. A functional indole oxygenase, termed IndA, was identified in the cluster, and its catalytic efficiency was higher than those of previously reported indole oxidation enzymes. Furthermore, the indole downstream metabolism was found to proceed via the atypical CoA-thioester pathway rather than conventional gentisate and salicylate pathways. This unusual pathway was catalyzed by a conserved 2-aminobenzoyl-CoA gene cluster, among which the 2-aminobenzoyl-CoA ligase initiated anthranilate transformation. This study unveils the genetic determinants of indole biotransformation and will provide new insights into our understanding of indole biodegradation in natural environments and its functional studies. This article is protected by copyright. All rights reserved. We report the indole biotransformation pathway and genetic determinants in an indole-degrading strain Cupriavidus sp. SHE. An efficient indole oxygenase encoded by indA from an up-regulated ind gene cluster is responsible for indole oxidation, and degradation of the intermediate anthranilate is catalyzed by an unusual 2-aminobenzoyl-CoA gene cluster. Both of the gene clusters are spread in various bacterial genera, highlighting their important roles in indole biotransformation.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1111/mmi.13852

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