3 years ago

Protein Modification by Endogenously Generated Lipid Electrophiles: Mitochondria as the Source and Target

Protein Modification by Endogenously Generated Lipid Electrophiles: Mitochondria as the Source and Target
William N. Beavers, Bing Zhang, Xiaojing Wang, Salisha Hill, James J. Galligan, Ned A. Porter, Michelle M. Mitchener, Lawrence J. Marnett, H. Alex Brown, Kristie L. Rose, Keri A. Tallman, Carol A. Rouzer, Pavlina T. Ivanova, Connor R. Lamberson
Determining the impact of lipid electrophile-mediated protein damage that occurs during oxidative stress requires a comprehensive analysis of electrophile targets adducted under pathophysiological conditions. Incorporation of ω-alkynyl linoleic acid into the phospholipids of macrophages prior to activation by Kdo2-lipid A, followed by protein extraction, click chemistry, and streptavidin affinity capture, enabled a systems-level survey of proteins adducted by lipid electrophiles generated endogenously during the inflammatory response. Results revealed a dramatic enrichment for membrane and mitochondrial proteins as targets for adduction. A marked decrease in adduction in the presence of MitoTEMPO demonstrated a primary role for mitochondrial superoxide in electrophile generation and indicated an important role for mitochondria as both a source and target of lipid electrophiles, a finding that has not been revealed by prior studies using exogenously provided electrophiles.

Publisher URL: http://dx.doi.org/10.1021/acschembio.7b00480

DOI: 10.1021/acschembio.7b00480

You might also like
Never Miss Important Research

Researcher is an app designed by academics, for academics. Create a personalised feed in two minutes.
Choose from over 15,000 academics journals covering ten research areas then let Researcher deliver you papers tailored to your interests each day.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.