3 years ago

crRNAs in Porphyromonas gingivalis CRISPR-Cas I-C system.

Krochmal, Staniec, Rodriguez Martinez, Pyrc, Burmistrz
The CRISPR-Cas (clustered regularly interspaced short palindromic repeats - CRISPR associated) system is unique to prokaryotes and provides the majority of bacteria and archaea with immunity against nucleic acids of foreign origin. crRNAs are the key element of this system, since they are responsible for its selectivity and effectiveness. Typical crRNAs consist of a spacer sequence flanked with 5' and 3' handles originating from repeat sequences that are important for recognition of these small RNAs by the Cas machinery. Herein, we studied the type I-C CRISPR-Cas system in Porphyromonas gingivalis, a human pathogen associated with periodontitis, rheumatoid arthritis, cardiovascular disease, and aspiration pneumonia. We demonstrated the importance of the 5' handle for crRNA recognition by the effector complex and consequently activity, as well as secondary trimming of the 3' handle, which was not affected by modifications of the repeat sequence.

Publisher URL: http://doi.org/10.1128/JB.00275-17

DOI: 10.1128/JB.00275-17

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