3 years ago

Daylight length-dependent translocation of VIVID photoreceptor in cells and its essential role in conidiation and virulence of Beauveria bassiana

Sheng-Hua Ying, Chong-Tao Guo, Sen-Miao Tong, Ming-Guang Feng, An-Xue Zhang
The fungal insect pathogen Beauveria bassiana has the blue-light photoreceptor VIVID (VVD) but lacks a pigmentation pattern to trace its light responses. Here we show that the fungal vvd is transcriptionally expressed, and linked to other blue/red photoreceptor genes, in a daylight length-dependent manner. GFP-tagged VVD fusion protein was localized to periphery, cytoplasm and vacuoles of hyphal cells in light/dark (L:D) cycles of 24:0 and 16:8 and aggregated in cytoplasm with shortening daylight until transfer into nuclei in full darkness. Deletion of vvd caused more reduced (91%) conidiation capacity in L:D 12:12 cycle of blue light (450/480 nm) than of yellow-to-red (540–760 nm) and white lights (∼70%). The conidiation defect worsened with shortened daylight in different L:D cycles of white light, coinciding well with drastic repression of key activator genes in central development pathway. Intriguingly, the deletion mutant displayed blocked secretion of cuticle-degrading Pr1 proteases, retarded dimorphic transition in insect haemocoel, and hence a lethal action twice longer than those for control strains against Galleria mellonella regardless of the infection passing or bypassing insect cuticle. Conclusively, VVD sustains normal conidiation in a daylight length-dependent manner and acts as a vital virulence factor in B. bassiana. This article is protected by copyright. All rights reserved.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1111/1462-2920.13951

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