5 years ago

Nonclassical Kinetics of Clonal yet Heterogeneous Enzymes

Nonclassical Kinetics of Clonal yet Heterogeneous Enzymes
Jaeyoung Sung, Ji-Hyun Kim, Hye Ran Koh, In-Chun Jeong, Seong Jun Park, Sanggeun Song
Enzyme-to-enzyme variation in the catalytic rate is ubiquitous among single enzymes created from the same genetic information, which persists over the lifetimes of living cells. Despite advances in single-enzyme technologies, the lack of an enzyme reaction model accounting for the heterogeneous activity of single enzymes has hindered a quantitative understanding of the nonclassical stochastic outcome of single enzyme systems. Here we present a new statistical kinetics and exactly solvable models for clonal yet heterogeneous enzymes with possibly nonergodic state dynamics and state-dependent reactivity, which enable a quantitative understanding of modern single-enzyme experimental results for the mean and fluctuation in the number of product molecules created by single enzymes. We also propose a new experimental measure of the heterogeneity and nonergodicity for a system of enzymes.

Publisher URL: http://dx.doi.org/10.1021/acs.jpclett.7b01218

DOI: 10.1021/acs.jpclett.7b01218

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