A 3-D Tail Explant Culture to Study Vertebrate Segmentation in Zebrafish

3 years ago

A 3-D Tail Explant Culture to Study Vertebrate Segmentation in Zebrafish

Here, we present the protocol for 3-D tissue culture of the zebrafish posterior body axis, enabling live study of vertebrate segmentation. This explant model provides control over axis elongation, alteration of morphogen sources, and subcellular resolution tissue-level live imaging.

Publisher URL: http://www.jove.com/v/61981/a-3-d-tail-explant-culture-to-study-vertebrate-segmentation

DOI: 2888.14084.f8113973-2a68-4863-a361-b0ee6a8b2d3b.1625118561

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