3 years ago

The Spore Coat Protein CotE Facilitates Host Colonisation by Clostridiumdifficile.

Ferreira, Wilkinson, Hong, Zentek, Hitri, Soloviev, Hosseini, Cutting, Vahjen, Anwar
Clostridium difficile infection (CDI) is an important hospital-acquired infection resulting from the germination of spores in the intestine as a consequence of antibiotic-mediated dysbiosis of the gut microbiota. Key to this is CotE, a protein displayed on the spore surface and carrying two functional elements, an N-terminal peroxiredoxin and a C-terminal chitinase domain. Using isogenic mutants we show in vitro and ex vivo that CotE enables binding of spores to mucus by direct interaction with mucin and contributes to its degradation. In animal models of CDI we show that when CotE is absent both colonisation and virulence was markedly reduced. We demonstrate here that the attachment of spores to the intestine is essential in the development of CDI. Spores are usually regarded as biochemically dormant but our findings demonstrate that rather than being simply agents of transmission and dissemination spores directly contribute to the establishment and promotion of disease.

Publisher URL: http://doi.org/10.1093/infdis/jix488

DOI: 10.1093/infdis/jix488

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