ACS Synthetic Biology
ACS Synthetic Biology
3 years ago

Multiple T-DNA Delivery to Plants Using Novel Mini Binary Vectors with Compatible Replication Origins

Multiple T-DNA Delivery to Plants Using Novel Mini Binary Vectors with Compatible Replication Origins
Joan Miquel Bernabé-Orts, Carmen Simón-Mateo, Araíz Gallo, Diego Orzaez, Fabio Pasin, Juan Antonio García, Leonor C. Bedoya
Improved plants are necessary to meet human needs. Agrobacterium-mediated transformation is the most common method used to rewire plant capabilities. For plant gene delivery, DNA constructs are assembled into binary T-DNA vectors that rely on broad host range origins for bacterial replication. Here we present pLX vectors, a set of mini binary T-DNA plasmids suitable for Type IIS restriction endonuclease- and overlap-based assembly methods. pLX vectors include replicons from compatible broad host range plasmids. Simultaneous usage of pBBR1- and RK2-based pLX vectors in a two-plasmid/one-Agrobacterium strain strategy allowed multigene delivery to plants. Adoption of pLX vectors will facilitate routine plant transformations and targeted mutagenesis, as well as complex part and circuit characterization.

Publisher URL: http://dx.doi.org/10.1021/acssynbio.6b00354

DOI: 10.1021/acssynbio.6b00354

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