3 years ago

Target of rapamycin complex 1 and Tap42-Associated Phosphatases Are Required for Sensing Changes in Nitrogen Conditions in the Yeast Saccharomyces cerevisiae

Target of rapamycin complex 1 and Tap42-Associated Phosphatases Are Required for Sensing Changes in Nitrogen Conditions in the Yeast Saccharomyces cerevisiae
Shaoxian Chen, Mingming Zhong, Gonghong Yan, Yong Jiang, Yin Zheng, Tong Jiang, Yu Jiang, Sichi Liu, Wenjie Yuan, Jinmei Li
In yeast target of rapamycin complex 1 (TORC1) and Tap42-associated phosphatases regulate expression of genes involved in nitrogen limitation response and the nitrogen discrimination pathway. However, it remains unclear whether TORC1 and the phosphatases are required for sensing nitrogen conditions. Utilizing temperature sensitive mutants of tor2 and tap42, we examined the role of TORC1 and Tap42 in nuclear entry of Gln3, a key transcription factor in yeast nitrogen metabolism, in response to changes in nitrogen conditions. Our data show that TORC1 is essential for Gln3 nuclear entry upon nitrogen limitation and downshift in nitrogen quality. However, Tap42-associated phosphatases are required only under nitrogen limitation condition. In cells grown in poor nitrogen medium, the nitrogen permease reactivator kinase (Npr1) inhibits TORC1 activity and alters its association with Tap42, rendering Tap42-associated phosphatases unresponsive to nitrogen limitation. These findings demonstrate a direct role for TORC1 and Tap42-associated phosphatases in sensing nitrogen conditions and unveil an Npr1-dependent mechanism that controls TORC1 and the phosphatases in response to changes in nitrogen quality. This article is protected by copyright. All rights reserved. This study shows that target of rapamycin complex 1 (TORC1) and Tap42 are directly involved in sensing changes in nitrogen conditions and their activities are inhibited by the Npr1 kinase in response to downshift in nitrogen quality.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1111/mmi.13858

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