3 years ago

Scalable Device for Automated Microbial Electroporation in a Digital Microfluidic Platform

Scalable Device for Automated Microbial Electroporation in a Digital Microfluidic Platform
Richard B. Fair, George M. Church, Matthew W. Royal, Frederic Vigneault, Peter B. Griffin, Liji Chen, Andrew C. Madison, Mark Horowitz
Electrowetting-on-dielectric (EWD) digital microfluidic laboratory-on-a-chip platforms demonstrate excellent performance in automating labor-intensive protocols. When coupled with an on-chip electroporation capability, these systems hold promise for streamlining cumbersome processes such as multiplex automated genome engineering (MAGE). We integrated a single Ti:Au electroporation electrode into an otherwise standard parallel-plate EWD geometry to enable high-efficiency transformation of Escherichia coli with reporter plasmid DNA in a 200 nL droplet. Test devices exhibited robust operation with more than 10 transformation experiments performed per device without cross-contamination or failure. Despite intrinsic electric-field nonuniformity present in the EP/EWD device, the peak on-chip transformation efficiency was measured to be 8.6 ± 1.0 × 108 cfu·μg–1 for an average applied electric field strength of 2.25 ± 0.50 kV·mm–1. Cell survival and transformation fractions at this electroporation pulse strength were found to be 1.5 ± 0.3 and 2.3 ± 0.1%, respectively. Our work expands the EWD toolkit to include on-chip microbial electroporation and opens the possibility of scaling advanced genome engineering methods, like MAGE, into the submicroliter regime.

Publisher URL: http://dx.doi.org/10.1021/acssynbio.7b00007

DOI: 10.1021/acssynbio.7b00007

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