5 years ago

Repurposing a Two-Component System-Based Biosensor for the Killing of Vibrio cholerae

Repurposing a Two-Component System-Based Biosensor for the Killing of Vibrio cholerae
Maciej B. Holowko, Jing Wui Yeoh, Chueh Loo Poh, Premkumar Jayaraman, Sierin Lim
New strategies to control cholera are urgently needed. This study develops an in vitro proof-of-concept sense-and-kill system in a wild-type Escherichia coli strain to target the causative pathogen Vibrio cholerae using a synthetic biology approach. Our engineered E. coli specifically detects V. cholerae via its quorum-sensing molecule CAI-1 and responds by expressing the lysis protein YebF-Art-085, thereby self-lysing to release the killing protein Art-085 to kill V. cholerae. For this report, we individually characterized YebF-Art-085 and Art-085 expression and their activities when coupled to our previously developed V. cholerae biosensing circuit. We show that, in the presence of V. cholerae supernatant, the final integrated sense-and-kill system in our engineered E. coli can effectively inhibit the growth of V. cholerae cells. This work represents the first step toward a novel probiotic treatment modality that could potentially prevent and treat cholera in the future.

Publisher URL: http://dx.doi.org/10.1021/acssynbio.7b00058

DOI: 10.1021/acssynbio.7b00058

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