5 years ago

Designing Uniquely Addressable Bio-orthogonal Synthetic Scaffolds for DNA and RNA Origami

Designing Uniquely Addressable Bio-orthogonal Synthetic Scaffolds for DNA and RNA Origami
Annunziata Lopiccolo, Jerzy Kozyra, Ulrich Stimming, Harold Fellermann, Alessandro Ceccarelli, Emanuela Torelli, Natalio Krasnogor, Jing-Ying Gu
Nanotechnology and synthetic biology are rapidly converging, with DNA origami being one of the leading bridging technologies. DNA origami was shown to work well in a wide array of biotic environments. However, the large majority of extant DNA origami scaffolds utilize bacteriophages or plasmid sequences thus severely limiting its future applicability as a bio-orthogonal nanotechnology platform. In this paper we present the design of biologically inert (i.e., “bio-orthogonal”) origami scaffolds. The synthetic scaffolds have the additional advantage of being uniquely addressable (unlike biologically derived ones) and hence are better optimized for high-yield folding. We demonstrate our fully synthetic scaffold design with both DNA and RNA origamis and describe a protocol to produce these bio-orthogonal and uniquely addressable origami scaffolds.

Publisher URL: http://dx.doi.org/10.1021/acssynbio.6b00271

DOI: 10.1021/acssynbio.6b00271

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