3 years ago

Ru(bpy)32+ Incorporated Luminescent Polymer Dots: Double-Enhanced Electrochemiluminescence for Detection of Single-Nucleotide Polymorphism

Ru(bpy)32+ Incorporated Luminescent Polymer Dots: Double-Enhanced Electrochemiluminescence for Detection of Single-Nucleotide Polymorphism
Huangxian Ju, Jianping Lei, Yaqiang Feng, Feng Sun, Ningning Wang
Encapsulating a large amount of electrochemiluminescence (ECL) active molecules into one nanoparticle for single-target recognition can greatly improve the sensitivity of the ECL assay. This work used luminescent conjugated polymer as a carrier to synthesize Ru(bpy)32+-doped polymer dots (RuPdots). The RuPdots with an average size of 20 nm and encapsulation of about 1354 Ru(bpy)32+ showed greatly enhanced ECL emission. Using tripropylamine as a coreactant, the resonance energy transfer from the excited polymer dots to the encapsulated Ru(bpy)32+ was demonstrated, which led to a 15.7-fold higher ECL emission than that of Ru(bpy)32+ with the same amount at the gold electrode. By coupling the double-enhanced ECL signal of this nanostructure with the specific ligase detection reaction, a sensitive ECL biosensor was constructed for specific detection of a single-nucleotide polymorphism (SNP). Using mutant KRAS gene as a model target, the ECL SNP detection method showed a linear range from 1 fM to 1 nM and a detection limit of 0.8 fM. The designed RuPdots provided a novel signal amplification strategy and could be used as a universal label for ultrasensitive ECL biodetection.

Publisher URL: http://dx.doi.org/10.1021/acs.analchem.7b01603

DOI: 10.1021/acs.analchem.7b01603

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.