5 years ago

Combined transcriptomic and proteomic analysis constructs a new model for light-induced anthocyanin biosynthesis in eggplant (Solanum melongena L.)

Lu Zhou, Li Ren, Yang Liu, Mingmin Jiang, Jing Li, Huoying Chen, Yongjun He, Zhen Gao
Light is a key environmental factor affecting anthocyanin biosynthesis. Our previous study demonstrated that ‘Lanshan Hexian’ is a light-sensitive eggplant cultivar, but its regulatory mechanism is unknown. Here, delphinidin-3-[4-(cis-p-coumaroyl)-rhamnosyl-glucopyranoside]-5-glucopyranoside and delphinidin-3-[4-(trans-p-coumaroyl)-rhamnosyl-glucopyranoside]-5-glucopyranoside were identified as the main anthocyanin components in ‘Lanshan Hexian’ by ultra-performance liquid chromatography-tandem mass spectrometry. Three time points of anthocyanin accumulation, including the start point (0 d), fastest point (5 d) and highest point (12 d), were investigated by using RNA-seq and iTRAQ technology. The corresponding correlation coefficients of differentially expressed genes and differentially expressed proteins were 0.6936, 0.2332 and 0.6672. Anthocyanin biosynthesis was a significantly enriched pathway, and CHI, F3H, 3GT, 5GT and HY5 were regulated at both transcriptional and translational levels. Moreover, some transcription factors and photoreceptors may participate in light-induced anthocyanin biosynthesis like the known transcription factors MYB113 and TT8. The transient expression assay indicated that SmMYB35, SmMYB44 and a SmMYB86 isoform might involve in the light-induced anthocyanin biosynthesis pathway. Finally, a regulatory model for light-induced anthocyanin biosynthesis in eggplant was constructed. Our work provides a new direction for the study of the molecular mechanisms of light-induced anthocyanin biosynthesis in eggplant.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1111/pce.13074

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