5 years ago

Copper Oxide Nanoparticles Induce Lysogenic Bacteriophage and Metal-Resistance Genes in Pseudomonas aeruginosa PAO1

Copper Oxide Nanoparticles Induce Lysogenic Bacteriophage and Metal-Resistance Genes in Pseudomonas aeruginosa PAO1
Jianhua Guo, Philip L Bond, Zhiguo Yuan, Willy Verstraete, Ji Lu, Shu-Hong Gao
The intensive use of metal-based nanoparticles results in their continuous release into the environment, leading to potential risks for human health and microbial ecosystems. Although previous studies have indicated that nanoparticles may be toxic to microorganisms, there is a scarcity of data available to assess the underlying molecular mechanisms of inhibitory and biocidal effects of nanoparticles on microorganisms. This study used physiological experiments, microscopy, live/dead staining, and the genome-wide RNA sequencing to investigate the multiple responses of Pseudomonas aeruginosa to the exposure of copper oxide nanoparticles (CuO NPs). The results for the first time show that CuO NPs induce lysogenic bacteriophage, which might render defective within a bacterial host. The presence of CuO NPs causes nitrite accumulation and great increases in N2O emissions. Respiration is likely inhibited as denitrification activity is depleted in terms of decreased transcript levels of most denitrification genes. Meanwhile, CuO NPs exposure significantly up-regulated gene expression for those coding for copper resistance, resistance-nodulation-division, P-type ATPase efflux, and cation diffusion facilitator transporters. Our findings offer insights into the interaction between environmental bacteria and CuO NPs at the transcriptional level and, thus, improve our understanding of potential risks of nanoparticles on microbial ecosystems and public health.

Publisher URL: http://dx.doi.org/10.1021/acsami.7b06433

DOI: 10.1021/acsami.7b06433

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.