4 years ago

Enhanced Antibacterial Properties of Self-Assembling Peptide Amphiphiles Functionalized with Heparin-Binding Cardin-Motifs

Enhanced Antibacterial Properties of Self-Assembling Peptide Amphiphiles Functionalized with Heparin-Binding Cardin-Motifs
Keerthana Subramanian, Thomas J. Webster, Mian Wang, Run Chang
The emergence of antibiotic resistance in bacteria has caused many healthcare problems and social burdens. In this study, a type of self-assembled peptide amphiphiles (PA) functionalized with a heparin-binding Cardin-motif peptide (sequence (AKKARK)2) has been designed to combat bacterial drug resistance. Above the critical micelle concentration (CMC) at 45 μM, these amphiphilic Cardin antimicrobial peptide (ACA-PA) can self-assemble into cylindrical supramolecular structures (7–10 nm in diameter) via hydrophobic interactions and β-sheet secondary conformation. The ACA-PA displays excellent antibacterial properties against both Gram-positive and Gram-negative bacteria. This work also demonstrates the effects of molecular self-assembly on antibacterial activity of peptide amphiphiles. The ACA-PA exhibits antibacterial activity on Gram-positive bacteria in a dose-dependent manner, but in the case of Gram-negative bacteria, the antibacterial potency of ACA-PA is remarkably enhanced at concentrations above the CMC. The ACA-PA has been shown to cause bacterial cytoplasmic leakage, causing localized membrane disruption in Gram-positive bacteria and blisters on disorganized membranes of Gram-negative bacteria. Therefore, these peptide-based nanoparticles have promising potential as antimicrobial agents without resorting to the use of antibiotics, and, thus, should be further studied for a wide range of biomaterial applications.

Publisher URL: http://dx.doi.org/10.1021/acsami.7b07506

DOI: 10.1021/acsami.7b07506

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.