5 years ago

The effects of modified atmosphere packaging and enzyme inhibitors on protein oxidation of tilapia muscle during iced storage

The objectives of this study were to investigate whether modified atmosphere packaging (MAP) and inhibitors of calpain, caspase-3, and cathepsins can reduce protein oxidation of tilapia muscle during iced storage. Parameters such as protein solubility, total carbonyl and sulfhydryl content as well as electrophoretic pattern of myofibrillar proteins from tilapia muscle were examined. We found that protein solubility, sulfhydryl group contents, and electrophoretic band intensities of myosin heavy chain and actin in MAP samples were significantly higher, whereas carbonyl content was significant lower than those in AP samples (P < 0.05). The extent of degradation in myosin heavy chain and actin in muscle tissue stored in AP was higher than that in MAP, suggesting that the latter was a better method to reduce protein oxidation during iced storage. After storage for 14 d with different enzyme inhibitors, carbonyl content, sulfhydryl content, and protein solubility in muscle samples were significantly affected by treatment (P < 0.05 for all three measures). The best preservation effect, similar to that of MAP, was achieved by the inclusion of the calpain inhibitor MDL-28170.

Publisher URL: www.sciencedirect.com/science

DOI: S0023643817306163

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.