Journal of the American Chemical Society
Journal of the American Chemical Society
5 years ago

Structural Basis for Aza-Glycine Stabilization of Collagen

Structural Basis for Aza-Glycine Stabilization of Collagen
David M. Chenoweth, Yitao Zhang, Yang Hai, Alexander J. Kasznel
Previously, we have demonstrated that replacement of the strictly conserved glycine in collagen with aza-glycine provides a general solution for stabilizing triple helical collagen peptides.1,2 The additional hydrogen bond and conformational constraints provided by aza-glycine increases the thermal stability and rate of folding in collagen peptides composed of Pro-Hyp-Gly triplet repeats, allowing for truncation to the smallest self-assembling peptide systems observed to date. Here we show that aza-glycine substitution enhances the stability of an arginine-containing collagen peptide and provide a structural basis for this stabilization with an atomic resolution crystal structure. These results demonstrate that a single nitrogen atom substitution for a glycine alpha-carbon increases the peptide’s triple helix melting temperature by 8.6 °C. Furthermore, we provide the first structural basis for stabilization of triple helical collagen peptides containing aza-glycine and we demonstrate that minimal alteration to the peptide backbone conformation occurs with aza-glycine incorporation.

Publisher URL: http://dx.doi.org/10.1021/jacs.7b03398

DOI: 10.1021/jacs.7b03398

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