3 years ago

Silencing of copine genes confers common wheat enhanced resistance to powdery mildew

Yuan Ding, He Liu, Baohong Zou, Jian Hua
Powdery mildew, caused by the biotrophic fungal pathogen Blumeria graminis f. sp. tritici (Bgt), is a major threat for the production of wheat (Triticum aestivum). It is of great importance to identify new resistance genes for the generation of Bgt resistant or tolerant wheat varieties. Here, we show that wheat copine genes TaBON1 and TaBON3 negatively regulate wheat disease resistance to Bgt. Two copies of TaBON1 and three copies of TaBON3 located on chromosomes 6AS, 6BL, 1AL, 1BL and 1DL, respectively, were identified from the current common wheat genome sequences. The expression of TaBON1 and TaBON3 are responsive to both pathogen infection and temperature changes. Knocking down of TaBON1 or TaBON3 by virus induced gene silencing (VIGS) each induces upregulation of defense responses in wheat. These TaBON1 or TaBON3 silenced plants exhibit enhanced wheat disease resistance to Bgt accompanied by higher accumulation of hydrogen peroxide and heightened cell death. In addition, high temperature has little effect on the upregulation of defense response genes conferred by silencing TaBON1 or TaBON3. Our study shows a conserved function of the plant copine genes in plant immunity and provides new genetic resources for improving resistance to powdery mildew in wheat. This article is protected by copyright. All rights reserved.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1111/mpp.12617

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