Molecular Plant Pathology
Molecular Plant Pathology
5 years ago

The TNL gene Rdr1 confers broad-spectrum resistance to Diplocarpon rosae

Ina Menz, Jannis Straube, Thomas Debener, Marcus Linde
Black spot disease, which is caused by the ascomycete Diplocarpon rosae, is the most severe disease in field-grown roses in temperate regions and has been distributed worldwide, likely along with commercial cultivars. Here, we present data supporting that muRdr1A is the active Rdr1 gene, a single-dominant TIR-NBS-LRR (TNL) type resistance gene against black spot disease that acts against a broad range of pathogenic isolates independently of the genetic background of the host genotype. The molecular analyses revealed that compared to the original donor genotype, the multiple integrations that are found in the primary transgenic clone segregate into different integration patterns in its sexual progeny and do not show any sign of overexpression. Rdr1 provides resistance to 13 different single-spore isolates belonging to six different races and broad field mixtures of conidia; thus far, Rdr1 is only overcome by two races. The expression of muRdr1A, the active Rdr1 gene, leads to interaction patterns that are identical in the transgenic clones and the non-transgenic original donor genotype. This finding indicates that the interacting Avr factor on the pathogen side must be widespread among the pathogen populations and may have a central function in the rose-black spot interaction. Therefore, the Rdr1 gene, pyramided with only a few other R-genes by sexual crosses, might be useful for breeding roses that are resistant to black spot because the spread of new pathogenic races of the fungus appears to be slow. This article is protected by copyright. All rights reserved.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1111/mpp.12589

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