3 years ago

Dodecyl-β-melibioside Detergent Micelles as a Medium for Membrane Proteins

Dodecyl-β-melibioside Detergent Micelles as a Medium for Membrane Proteins
Ritesh Mittal, Zhenwei Lu, Geoffrey C. Li, Charles R. Sanders, James M. Hutchison, Benjamin Travis, Catherine L. Deatherage
There remains a need for new non-ionic detergents that are suitable for use in biochemical and biophysical studies of membrane proteins. Here we explore the properties of n-dodecyl-β-melibioside (β-DDMB) micelles as a medium for membrane proteins. Melibiose is d-galactose-α(1→6)-d-glucose. Light scattering showed the β-DDMB micelle to be roughly 30 kDa smaller than micelles formed by the commonly used n-dodecyl-β-maltoside (β-DDM). β-DDMB stabilized diacylglycerol kinase (DAGK) against thermal inactivation. Moreover, activity assays conducted using aliquots of DAGK purified into β-DDMB yielded activities that were 40% higher than those of DAGK purified into β-DDM. β-DDMB yielded similar or better TROSY-HSQC NMR spectra for two single-pass membrane proteins and the tetraspan membrane protein peripheral myelin protein 22. β-DDMB appears be a useful addition to the toolbox of non-ionic detergents available for membrane protein research.

Publisher URL: http://dx.doi.org/10.1021/acs.biochem.7b00810

DOI: 10.1021/acs.biochem.7b00810

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