Journal of Biophotonics
Journal of Biophotonics
5 years ago

Polarimetric imaging of biological tissues based on the indices of polarimetric purity

Enric Garcia-Caurel, Ignacio Moreno, Juan Carlos Escalera, Carla Rodríguez, Adrià Sansa, Emilio González, Irene Estévez, Angel Lizana, José J. Gil, Albert Van Eeckout, Juan Campos
We highlight the interest of using the Indices of Polarimetric Purity (IPPs) to the inspection of biological tissues. The IPPs were recently proposed in the literature and they result in a further synthetization of the depolarizing properties of samples. Compared with standard polarimetric images of biological samples, IPPs based images lead to larger image contrast of some biological structures and to a further physical interpretation of the depolarizing mechanisms inherent to the samples. In addition, unlike other methods, their calculation do not require advanced algebraic operations (as is the case of polar decompositions), and they result in three indicators of easy implementation. We also propose a pseudo-coloured encoding of the IPPs information that leads to an improved visualization of samples. This last technique opens the possibility of tailored adjustment of tissues contrast by using customized pseudo-coloured images. The potential of the IPPs approach is experimentally highlighted along the manuscript by studying three different ex-vivo samples. A significant image contrast enhancement is obtained by using the IPPs based methods, compared to standard polarimetric images.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/jbio.201700189

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