3 years ago

Investigation of binding properties of two ethidium derivatives with serum albumins: spectral and computational approach

Elmas Gökoğlu, Nuriye Akbay, Zeynel Seferoğlu, Tugba Taskin Tok

The interaction mechanisms of two ethidium derivatives, 3,8-dibenzoylamino-5-ethyl-6-phenylphenantridinium chloride (E2) and 3,8-diphenylacetylamino-5-ethyl-6-phenylphenantridinium chloride (E3) with serum albumins (BSA and HSA) have been investigated by a combined experimental and computational approach. Fluorescence quenching and UV–vis results revealed that the interaction of derivatives with albumins resulted in formation of ground-state complexes and the obtained Stern–Volmer quenching constants designate the presence of a static component in the quenching mechanisms. Thermodynamic parameters (ΔH and ΔS values) point out the ionic interactions play the major role in E2-BSA, E2-HSA and E3-HSA complexes. The van der Waals interactions are dominant forces in E3-BSA complex. Moreover, the obtained results in this study were supported with computational analyzes which have same tendency.

Publisher URL: http://www.tandfonline.com/doi/full/10.1080/07391102.2017.1380536

DOI: 10.1080/07391102.2017.1380536

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