3 years ago

Effect of Pulsed Electric Fields (PEF) on Accumulation of Magnesium in Lactobacillus rhamnosus B 442 Cells

Małgorzata Góral, Urszula Pankiewicz

Abstract

The aim of this study was to determine the effect of pulsed electric fields (PEF) on accumulation of magnesium ions in Lactobacillus rhamnosus B 442 cells. Under optimized conditions, this is, on 15 min exposure of the 20 h grown culture to PEF of the 2.0 kV/cm and 20 µs pulse width at concentration 400 μg Mg2+/mL medium, accumulation of magnesium in the biomass reached maximum 4.28 mg/g d.m. Optimization of PEF parameters caused an increase of magnesium concentration in the cells by 220% in comparison to the control not treated with PEF. Bacterial cell biomass enriched with Mg2+ may be an alternative for pharmacological supplementation applied in deficiency of this cation.

Publisher URL: https://link.springer.com/article/10.1007/s00232-017-9986-6

DOI: 10.1007/s00232-017-9986-6

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