3 years ago

Analysis of imidazoles and triazoles in biological samples after MicroExtraction by packed sorbent

Analysis of imidazoles and triazoles in biological samples after MicroExtraction by packed sorbent
Luisa Di Marzio, Sergio Menta, Elisa De Luca, Giuseppe Bellagamba, Gokhan Zengin, Marcello Locatelli, Cristina Campestre, Paolo Guglielmi, Simone Carradori, Christian Celia

Abstract

This paper reports the MEPS-HPLC-DAD method for the simultaneous determination of 12 azole drugs (bifonazole, butoconazole, clotrimazole, econazole, itraconazole, ketoconazole, miconazole, posaconazole, ravuconazole, terconazole, tioconazole and voriconazole) administered to treat different systemic and topical fungal infections, in biological samples. Azole drugs separation was performed in 36 min. The analytical method was validated in the ranges as follows: 0.02–5 μg mL−1 for ravuconazole; 0.2–5 μg mL−1 for terconazole; 0.05–5 μg mL−1 for the other compounds. Human plasma and urine were used as biological samples during the analysis, while benzyl-4-hydroxybenzoate was used as an internal standard. The precision (RSD%) and trueness (Bias%) values fulfill with International Guidelines requirements. To the best of our knowledge, this is the first HPLC-DAD procedure coupled to MEPS, which provides the simultaneous analysis of 12 azole drugs, available in the market, in human plasma and urine. Moreover, the method was successfully applied for the quantitative determination of two model drugs (itraconazole and miconazole) after oral administration in real samples.

Publisher URL: http://tandfonline.com/doi/full/10.1080/14756366.2017.1354858

DOI: 10.1080/14756366.2017.1354858

You might also like
Never Miss Important Research

Researcher is an app designed by academics, for academics. Create a personalised feed in two minutes.
Choose from over 15,000 academics journals covering ten research areas then let Researcher deliver you papers tailored to your interests each day.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.