Journal of Peptide Science
Journal of Peptide Science
5 years ago

The American cockroach peptide periplanetasin-4 inhibits Clostridium difficile toxin A-induced cell toxicities and inflammatory responses in the mouse gut

The American cockroach peptide periplanetasin-4 inhibits Clostridium difficile toxin A-induced cell toxicities and inflammatory responses in the mouse gut
Ji Hong, Jae Sam Hwang, Li Fang Lu, I Na Yoon, Jin Ku Kang, Peng Zhang, Ho Kim, Dae Hong Kim
Many reports have shown that crude extracts of the American cockroach have therapeutic effects on inflammation. In a previous study, our research group showed that an antimicrobial peptide (Periplanetasin-2) derived from the American cockroach via de novo transcriptome analysis inhibited apoptosis of human colonocytes and inflammatory responses of the mouse gut caused by Clostridium difficile toxin A. Here, we examined whether Periplanetasin-4 (Peri-4), another antimicrobial peptide identified via de novo transcriptome analysis of the American cockroach, could also inhibit the various toxicities induced by C. difficile toxin A. We found that Peri-4 significantly reduced the cell viability loss and cell apoptosis caused by toxin A in vitro. Peri-4 also ameliorated the severe inflammatory responses seen in the toxin A-induced mouse enteritis model, rescuing the villus disruption and interleukin-6 production induced by luminal injection of toxin A into the mouse gut. Mechanistically, we found that Peri-4 could reduce toxin A-induced reactive oxygen species production to inhibit the activations of p38MAPK and p21Cip1/Waf1, which are critical for the cell damages induced by toxin A. These results collectively suggest that the Peri-4 may be a potential therapeutic agent for treating toxin A-induced pseudomembranous colitis. Copyright © 2017 European Peptide Society and John Wiley & Sons, Ltd. We synthesized Periplanetasin-4 (Peri-4), an antimicrobial peptide identified via de novo transcriptome analysis of the American cockroach, tested its therapeutic effect on C. difficile toxin A-induced gut inflammation and found that Peri-4 inhibits cell viability loss and apoptosis induced by toxin A. Peri-4 also rescued toxin A-induced villus disruption and inflammation in the mouse gut. Insect derived peptide, Peri-4, may be a potent therapeutic agent for C. difficile toxin A-induced colitis.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/psc.3046

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